银杏愈伤组织培养基优化研究.pdf

银杏愈伤组织培养基优化研究

Title:OptimizationofGinkgobilobaCallusCultureandIts

ApplicationinWoundHealing

Abstract:

Ginkgobiloba,popularlyknownasthemaidenhairtree,isawell-

knownmedicinalplantwithseveraltherapeuticbenefits.Inthisstudy,

wefocusedonoptimizingthecultureconditionsforGinkgobilobacallus

formationandexploreditspotentialapplicationsinwoundhealing.A

rangeofphysiologicalandbiochemicalparameterswereexaminedto

evaluatethegrowthofcallustissue.Theresultsindicatethatan

optimizedtissueculturemediumplaysacrucialroleinpromotingcallus

initiation,proliferation,andregeneration.Furthermore,weinvestigated

theeffectsofGinkgobilobacallusextractonwoundhealingactivity

usinginvitroandinvivomodels.OurfindingssuggestthatGinkgo

bilobacallusculturehasgreatpotentialasanaturalsourceofwound

healingagents.

Introduction:

Ginkgobiloba,auniquedeciduoustreespecies,hasbeenusedin

traditionalmedicineforcenturiesduetoitsnumerouspharmacological

activities.Itcontainsrichsourcesofflavonoids,terpenoids,andother

bioactivecompoundsthatpossessantioxidant,anti-inflammatory,and

woundhealingproperties.Theabilitytoinducecallusformationinvitro

providesaplatformforlarge-scaleproductionofthesebioactive

compounds.Therefore,optimizingthecultureconditionsforGinkgo

bilobacallusformationiscrucialfordevelopingnewtherapeutic

approachesinwoundhealing.

Methods:

Thestudyinvolvedthepreparationofthetissueculturemedium

usingvariouscombinationsofplantgrowthregulators(PGRs)suchas

auxinsandcytokinins.Parameterssuchascallusinitiationfrequency,

growthrate,biomassaccumulation,andbiochemicalcompositionwere

assessedtodeterminetheoptimalmediumformulation.Calluscultures

wereestablishedbyinoculatingexplantsontothedevelopedmedium

a