the canine oral microbiome狗狗口腔微生物.pdf

The Canine Oral Microbiome 1,2 1 1 1 1 3 Floyd E. Dewhirst *, Erin A. Klein , Emily C. Thompson , Jessica M. Blanton , Tsute Chen , Lisa Milella , 4 4 5 4 Catherine M. F. Buckley , Ian J. Davis , Marie-Lousie Bennett , Zoe V. Marshall-Jones 1 Department of Molecular Genetics, The Forsyth Institute, Cambridge, Massachusetts, United States of America, 2 Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, United States of America, 3 The Veterinary Dental Surgery, Byfleet, United Kingdom, 4 WALTHAM Centre for Pet Nutrition, Melton Mowbray, United Kingdom, 5 Mars Pet Care Europe, Birstall, United Kingdom Abstract Determining the bacterial composition of the canine oral microbiome is of interest for two primary reasons. First, while the human oral microbiome has been well studied using molecular techniques, the oral microbiomes of other mammals have not been studied in equal depth using culture independent methods. This study allows a comparison of the number of bacterial taxa, based on 16S rRNA-gene sequence comparison, shared between humans and dogs, two divergent mammalian species. Second, canine oral bacteria are of interest to veterinary and human medical communities for understanding their roles in health and infectious diseases. The bacteria involved are mostly unnamed and not linked by 16S rRNA-gene sequence identity to a taxonomic scheme. This manuscript describes the analysis of 5,958 16S rRNA-gene sequences from 65 clone libraries. Full length 16S rRNA reference sequences have