solid-phase microextraction and the human fecal voc metabolome固相微萃取和人类粪便voc代谢物.pdf

Solid-Phase Microextraction and the Human Fecal VOC Metabolome Emma Dixon1,5, Cynthia Clubb1,5, Sara Pittman1,5, Larry Ammann1,5, Zeehasham Rasheed2,5, Nazia 4 4 3,5 2,5 1,5 Kazmi , Ali Keshavarzian , Pat Gillevet , Huzefa Rangwala , Robin D. Couch * 1 Department of Chemistry and Biochemistry, George Mason University, Manassas, Virginia, United States of America, 2 Department of Computer Science and Engineering, George Mason University, Fairfax, Virginia, United States of America, 3 Department of Environmental Science and Policy, George Mason University, Manassas, Virginia, United States of America, 4 Department of Medicine, Rush University Medical Center, Chicago, Illinois, United States of America, 5 The Microbiome Analysis Center, George Mason University, Manassas, Virginia, United States of America Abstract The diagnostic potential and health implications of volatile organic compounds (VOCs) present in human feces has begun to receive considerable attention. Headspace solid-phase microextraction (SPME) has greatly facilitated the isolation and analysis of VOCs from human feces. Pioneering human fecal VOC metabolomic investigations have utilized a single SPME fiber type for analyte extraction and analysis. However, we hypothesized that the multifarious nature of metabolites present in human feces dictates the use of several diverse SPME fiber coatings for more comprehensive metabolomic coverage. We report here an evaluation of eight different commercially available SPME fibers, in combination with both GC-MS and GC- FID, and identify the 50/30 mm CAR-DVB-PDMS, 85 mm CAR-PDMS, 65 mm DVB-PDMS, 7 mm PDMS, and 60 mm PEG SPME fibers as a minimal set of fibers appropriate for human fecal VOC metabolomics, collectively