酶联免疫吸附测定法实验报告(共4篇).doc
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酶联免疫吸附测定法实验报告(共4篇)
酶联免疫吸附测定法
酶联免疫吸附测定法（ELISA）
1. 定义 .......................................................................................................................................... 3
2. 原理 .......................................................................................................................................... 3
2.1
2.2 抗原抗体反应 ............................................................................................................... 3 免疫测定在临床检验中的应用 ................................................................................... 5
3. ELISA的类型 ............................................................................................................................. 5
3.1
3.2
3.3
3.4
3.5
3.6
3.7 双抗体夹心法测抗原： ............................................................................................... 6 双抗原夹心法测抗体 ................................................................................................... 6 间接法测抗体 ............................................................................................................... 6 竞争法测抗体 ............................................................................................................... 7 竞争性测抗原 ............................................................................................................... 7 捕获包被法测抗体 ....................................................................................................... 7 ABS-ELISA法 ................................................................................................................. 8
4. ELISA试剂的组成 ..................................................................................................................... 9
4.1
4.2
4.3
4.4
4.5
4.7
4.8
4.9
4.10
4.11
4.12
4.13
4.14 固相载体： ................................................................................................................... 9 包被的方式 ...................................................................................
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