脱氢酶电化学生物传感器的研究进展-分析化学.pdf

37 ( FENX I HUAXUE ) 4 2009 4 Ch inese Journa l of A na lytica l Chem istry 6 17 ~ 623 评述与进展 * 周敬丽 聂萍萍 郑海涛 张纪梅 (, 300160) 4 00 -( NAD+ ) ( NAD P+ ) , , , , , , 1 20 60 C lark , 50, , ; ( I, NAD+ ) + [ 1] ( II, NADP ) , ( g lu cose ox idase, GOx ) [ 2~ 9] ( H orserad ish P erox idase, H RP ) [ 10~ 12] , [ 13 ] NAD+ NADP+ 1 , 400 NAD+ NADP+ , ( a lcohol dehydrogenase, ADH ) ( lactate dehydrogenase, LDH ) + 1 NAD ( P) ( m a la te dehydrogenase, MDH ) F ig. 1 Chem ica l stru ctu re of n icotinam ide aden ine d inu c leotide ( g lutam ate dehydrogenase, G lDH ) + pho sph ate( NAD ( P) ) ( form ate dehydrogenase, FDH ) ( form aldehyde dehydrogenase, FALDH )-6- ( g lucose-6-phosphate dehydrogenase, G6PDH ) ( g lycero l dehydrogenase, G lyDH )3-( 3-hydroxybuty rate dehydro- genase, 3-HBDH ) + + Sub strate+ NAD ( P) NAD ( P ) H + H + P rodu ct ( 1) , NAD+ NADP+ NA DH NADPH, NAD ( P) + /NAD ( P) H , NADH NADPH, , [ 14, 15 ] 2008-07-04; 2008-12-06 ( N o. 06Y FJM JC 15000 ) * E-m ail zhenght999@ hotm ai.l com 37 618 , , , 2 2. 1 NAD ( P) H 20 80 , NA D ( P ) H NAD ( P ) H +