Robust Sequence Selection Method Used To Develop the FluChip Diagnostic Microarray for Infl.pdf

JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2006, p. 2857–2862 Vol. 44, No. 8 0095-1137/06/$08.00 0 doi:10.1128/JCM.00135-06 Copyright © 2006, American Society for Microbiology. All Rights Reserved. Robust Sequence Selection Method Used To Develop the FluChip Diagnostic Microarray for Influenza Virus Martin Mehlmann,1 1 1 1 1 † Erica D. Dawson, † Michael B. Townsend, James A. Smagala, Chad L. Moore, Catherine B. Smith,2 2 1 1,3 Nancy J. Cox, Robert D. Kuchta, * and Kathy L. Rowlen Department of Chemistry and Biochemistry, The University of Colorado at Boulder, UCB #215, Boulder, Colorado 80309 1 ; Influenza Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, Georgia 303332; and InDevR, LLC, 2100 Central Ave., Suite 106, Boulder, Colorado 803013 Received 20 January 2006/Returned for modification 10 April 2006/Accepted 14 May 2006 DNA microarrays have proven to be powerful tools for gene expression analyses and are becoming increas- ingly attractive for diagnostic applications, e.g., for virus identification and subtyping. The selection of appropriate sequences for use on a microarray poses a challenge, particularly for highly mutable organisms