选择性环氧合酶_2抑制剂筛选模型的验证.pdf

安 徽 医 药 AnhuiM ed ical and Pharmaceutical Journal 2009 Aug; 1 ( 8) 879 选择性环氧合酶2抑制剂筛选模型的验证 1 1 1 2 3 , , , , ( 1. 安徽医学高等专科学校病理教研室, 安徽, 合肥 230032; 2. 安徽医科大学 病理生理学教研室, 安徽 合肥 230032; 3. 军事医学科学院放射与辐射医学研究所, 北京 100850) : 2 2, W estern COX2 COX1 0. 5 g L- 1 LPS 6 h, 2, COX2; , COX1 COX2 : COX2; ; ; W estern Validation of the dependability of screeningmodels for selective cox2 inhibitors 1 1 2 2 3 PAN X i nzhu , WANG X i oqing , X IE Linlin , WANG S iy ing , WANG L in ( 1. Department of P athology, Anhuimedical Colleg e, H ef ei 230032, China; 2. Department of Pathophysiology, AnhuiM ed ical University, H ef ei 230032, China; 3. Institute of adiationM ed icine, Academy of M ilitaryM ed icalSciences, B eij ing 100850, China) Abstract: A im T o v lid te the depend bility of screen ing m odels by culture of r tm croph ges in vitro. M ethods The PGE2 w s de tected by us ing r d iomi m uno ss y in d ifferen t concentr tion of serum, stmi ul ting tmi e nd concentr tion of lipopolys cch ride( LPS). The t rget protein COX1 nd COX2 were detected byW estern b lotting in d ifferent scrum concentr tion, stmi ul ting tmi e nd concentr tion of LPS. Results M croph ges producem ore PGE2 fter stmi ul ted by LPS in concentr tion of 0. 5 g L- 1 for 6 hours, m e n wh ile, produced m ore COX2 th n others. There is not n obvious difference on COX1 betw een 0. 5% nd 10% FCS RPIM1640, but it decre ses visibly fter spirin dded to it. Conclusion The screen ing m odels c n be used for screen ing selective COX2 inhib itors of the ntiinfl mm tory drugs. K ey words: selective cyclooxygen se2 inh ib itors; m croph ges; screening models; western b