硫代乙酰胺肝硬化模型2010.pdf

MolBiolRep

DO10.1007/s11033-010-0281-1

Anaturalprocessofcirrhosisresolutionanddecelerationofliver

regenerationafterthioacetamidewithdrawalinaratmodel

KeGuJian-DongZhaoZhi-GangRen•••

Ning-YiMaSong-TaoLaiJianWang•••

JinLiuGuo-LiangJiang•

Received:15February2010/Accepted:2September2010

SpringerScience+BusinessMediaB.V.2010

AbstractCharacteristicsofthioacetamide(TAA)-inducedwithdecreasesofALT,AST,andALP,andincreaseofPA.

livercirrhosisinratwasobservedfor120daysafterTAATheresolutionofcirrhosiswasevidentbyhistological

withdrawalaspartoftheradiobiologicalstudyofpartialimprovementwithattenuationofcollagenfiberand

liverirradiationonTAA-inducedcirrhoticrats.ThenaturaldecreaseofTGF-b1IHCindex,andalsodecreaseoftri-

processfocusedoncirrhosisandregenerationwasrecordedchromestainandhydroxyprolinecontent.However,cir-

asabaselineconditionfortheinterpretationoftheoutcomerhosiswasstillexistedon120daysafterTAAwithdrawal.

ofthepartialliverirradiationstudy.CirrhosisinratswasSignificantdecelerationofliverregenerationwasdemon-

successfullyinducedbydrinking0.03%TAAwaterorallystratedwithTAAwithdrawal,evidencedbydecreaseofM

for29weekswithamodelingrateof96%.Afterestab-andPI,reducedexpressionofPCNAmRNAandPCNALI.

lishmentofthecirrhosismodel,theratswereobservedforInconclusion,uponTAAwithdrawalhepaticcirrhosiswas

120daysuponTAAwithdrawaltoinvestigatethedynamiccontinuouslyresolved,butpersistedupto120days,and

changesofcirrhosisandregeneration.Thefollowingliverregenerationwassignificantlydecelerated.

characteristicswereobserved:(1)Histologicalchanges;

(2)Liverfunctions;(3)Cirrhosis:trichromestain,quanti-KeywordsThioacetamideÁLivercirrhosisÁ

ficationofhydroxyprolineinhydrolysedlivertissueandLiverregeneration

TGF-b1;(4)Liverregeneration:liverindex,hepatocyte

mitoticindex(MI),hepatocyteproliferati