陕南水牛微卫星DNA遗传多样性研究.doc

陕南水牛微卫星DNA遗传多样性研究 王 斌1,2，昝林森1,3，杨彦杰4，钟 昕1，黄 磊1，王洪宝1,3 （1西北农林科技大学 动物科技学院，陕西 杨凌 712100；汉中职业技术学院 农林系，陕西 汉中 723000；国家肉牛改良中心，中国 杨凌 7121004；河南师范大学 生命科学学院，河南 新乡45300，选育程度较低,遗传一致性程度较大。 [关键词] 陕南水牛；微卫星；遗传多样性 [中图分类号] [文献标识码] A [文章编号] Study on genetic diversity of microsatellite DNA in Shaannan buffalo WANG Bin1,2 ,ZAN Lin-sen1,3,YANG Yan-jie4,ZHONG Xin1,HUANG Lei1,WANG Hong-bao1,3 (1 College of Animal Science and Technology，Northwest AF University，Yangling，Shaanxi 712100；2 AF Department，Hanzhong Vocation and Technology College，Hanzhong Shaanxi 723000,China； 3 National eef Cattle Improvement Center in China, Yangling,Shaanxi 712100,China ; 4 College of Life Sciences, Henan Normal University，Xinxiang，Henan 453007) Abstract:【Objective】To analys genetic diversity of four landraces buffalo in southern Shaanxi and to provide support for the protection and utilization of resources of buffalo species in southern Shaanxi.【Method】19Shaannan Buffalo（were selected from ChengguNanzheng,Ningqiang and Hanbin）were used to the genetic diversity,and counted allele frequency of each group, number of alleles, effective number of alleles (Ne), heterozygosity (H), between groups Neis standard genetic distance and the polymorphic information content (PIC), analysize genetic distance through cluster analysis.【Result】The results indicate that there are104 alleles and 8 alleles-specific at 10 microsatellite locus in 4 local buffalo populations. The allele frequenciesverage effective number of alleles and the average polymorphic information content ranging from 0.005 2－0.349 0; 4.017 4－13.146 9 and 0.700 7 － 0.893 2,meanwhile, the average heterozygosity range from 0.755 0 － 0.928 8.they were highly polymorphic. Cluster analysis showed that Chenggu groups first of all together with Nanzheng, followed with Ningqiang, Hanbin.【Conclution】10 pairs of microsatellite markers can be used as an effective genetic marker for the analysis of the genetic diversity of bu