凝胶渗透色谱净化烟叶有机磷拟除虫菊酯类农药残留的研究.pdf

中国农学通报 2011,27(05):269-273 Chinese Agricultural Science Bulletin 凝胶渗透色谱净化烟叶有机磷、拟除虫菊酯类 农药残留的研究 方敦煌，师君丽，宋春满 （云南省烟草农业科学研究院，云南玉溪653100） 摘 要：为确定凝胶渗透色谱（GPC ）净化烟草有机磷和拟除虫菊酯农药残留的最佳收集时间，提高烟草 有机磷和拟除虫菊酯农药残留的净化效果和回收率，进行了此项试验。采用添加3 个浓度梯度农药标 准品进行回收试验的方法，研究了烟叶中9 种有机磷农药和7 种拟除虫菊酯农药在GPC 柱上的流出规律。 结果表明，有机磷农药的最佳收集时间为26~68 min ，拟除虫菊酯类农药的最佳收集时间为26~42 min。有 机磷农药的回收率为80.28%~96.81% ，相对标准偏差为4.44%~8.74% ，拟除虫菊酯类农药回收率为 76.39%~97.95% ，相对标准偏差为2.69% ~7.58% 。GPC 净化烟草有机磷和拟除虫菊酯农药残留的最佳 收集时间与国家标准GB/T 13595 不同，主要是因为农药品种不同而造成的，因此最佳收集时间必须根 据具体试验来确定。 关键词：烟草；凝胶渗透色谱；农药残留 中图分类号：O657.7 文献标志码：A 论文编号：2010-2682 StudyonGPCClean-upofResiduesofOrganophosphorousPesticidesand SyntheticPyrethroidPesticidesinTobaccoLeaf Fang Dunhuang, Shi Junli, Song Chunman (YunnanAcademyofTobaccoAgriculturalSciences, Yuxi Yunnan 653100) Abstract: In order to determine the optimum collecting time of GPC clean-up organophosphorous and pyrethroid residue in tobacco, and improve of purificatory effect and recoveries of those pesticide residues, the test was in progress. According to recovery tests for 16 organophosphorous and pyrethroid pesticide standard spiked samples with 3 concentration gradients, elution behavior of from a gel permeation chromatography column (GPC) was determined for these pesticides in tobacco leaf. The results showed that the optimum collection time of 9 organophosphorous pesticides from GPC column was 26-68 min and that for 7 pyrethroid pesticides was 26-42 min. The recoveries of organophosphorous pesticides were 80.28%-96.81%, the relative standard deviation were 4.44%-8.74% , the recoveries of pyrethroid pesticides we