【2018年最新整理】仙客来组织培养研究.doc

仙客来组织培养的研究摘　要 目前，仙客来繁殖多用种子进行繁殖，且种子多为杂交种，不利于优良性状的保持。组织培养能够保持植物的优良性状并且有很高的繁殖率。对仙客来组织培养培养基配方的研究具有很高的基础理论价值和商业价值。利用组织培养的方法建立优系仙客来的无性繁殖体系，进行种苗的工厂化育苗生产，具有恢复和保持种性、快速大量繁殖优系品种的优势，可以从根本上解决仙客来生产上的种苗质量问题。 本试验以仙客来“国旗红”为研究对象，通过不同消毒时间处理仙客来叶片，确定最佳的消毒时间。通过加入不同的激素处理，筛选适宜的诱导培养基；比较活性炭和抗坏血酸两种方法哪种方法可以更好的防止仙客来褐化。 用升汞消毒6分钟时污染率最低；培养基MS+BA 2mg/L +NAA 1mg/L为诱导叶片形成愈伤组织的最佳培养基；MS+NAA mg/l + BA4 mg/l为愈伤组织的最佳增殖培养基。加入200mg/L的抗坏血酸可以有效防止外植体褐化。 关键词：仙客来； 组织培养； 褐化； 增殖 Abstract At present, Cyclamen persicum Mill the reproduction multipurpose seed carries on the reproduction, also the seed are many is a hybrid, is disadvantageous to the fine character maintenance. The tissue culture can maintain the plant the fine character and to have the very high reproduction rate. Has the very high basic theory value and the commercial value to Cyclamen persicum Mill the tissue culture base for mule research. Establishes superior is Cyclamen persicum Mill the vegetative reproduction systemusing the tissue culture method, carries on the seedling the factorization to grow seedlings the production, has restores and maintains plants the nature fast, massively reproduces superiorly is the variety superiority, may fundamentally solve Cyclamen persicum Mill in the production seedling quality problem. This experiment is red take Cyclamen persicum Mill the national flag as the research object, through the different disinfection time processing Cyclamen persicum Mill leaf blade, determines the best disinfection time. Through joins different hormone processing, the induction culture medium which screening is suitable; Better prevent brown Cyclamen persicum Mill compared with the activated charcoal and may the antics or buticacid two methods which method. Disinfects 6 minutes time pollution with the mercuric chloride rate to be lowest; Culture medium MS+BA 2mg/L +NAA 1mg/L is the induction injuries the organization the best culture medium. In MS+NAA mg/l +BA4 mg/l is the best multiplication culture medium. Joins 200mg/L the a