brugia malayi excretedsecreted proteins at the hostparasite interface stage- and gender-specific proteomic profiling与象皮病马来excretedsecreted蛋白质hostparasite接口阶段和性别蛋白质组分析.pdf

Brugia malayi Excreted/Secreted Proteins at the Host/ Parasite Interface: Stage- and Gender-Specific Proteomic Profiling 1 1 2 3 2 Sasisekhar Bennuru , Roshanak Semnani , Zhaojing Meng , Jose M. C. Ribeiro , Timothy D. Veenstra , Thomas B. Nutman1* 1 Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, National Cancer Institute at Frederick, Frederick, Maryland, United States of America, 3 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America Abstract Relatively little is known about the filarial proteins that interact with the human host. Although the filarial genome has recently been completed, protein profiles have been limited to only a few recombinants or purified proteins of interest. Here, we describe a large-scale proteomic analysis using microcapillary reverse-phase liquid chromatography-tandem-mass spectrometry to identify the excretory-secretory (ES) products of the L3, L3 to L4 molting ES, adult male, adult female, and microfilarial stages of the filarial parasite Brugia malayi. The analysis of the ES products from adult male, adult female, microfilariae (Mf), L3, and molting L3 larvae identified 852 proteins. Annotation suggests that the functional and component distribution was very similar across each of the stages studied; however, the Mf contributed a higher proportion to the total number of identified proteins than the other stag