study on suitability of kod dna polymerase for enzymatic production of artificial nucleic acids using basesugar modified nucleoside triphosphates研究的适用性kod dna聚合酶人工核酸酶生产使用修改后basesugar核苷三磷酸腺苷.pdf

Molecules 2010, 15, 8229-8240; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Study on Suitability of KOD DNA Polymerase for Enzymatic Production of Artificial Nucleic Acids Using Base/Sugar Modified Nucleoside Triphosphates Masayasu Kuwahara 1,*, Yuuki Takano 1, Yuuya Kasahara 1, Hiroki Nara 1, Hiroaki Ozaki 1, 1 2 3 Hiroaki Sawai , Akio Sugiyama and Satoshi Obika 1 Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515, Japan 2 Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., 10-24 Toyo-cho, Tsuruga, Fukui 914-0047, Japan 3 Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan * Author to whom correspondence should be addressed; E-Mail: kuwahara@chem-bio.gunma-u.ac.jp; Tel.: +81-277-30-1222; Fax: +81-277-30-1222. Received: 30 September 2010; in revised form: 6 November 2010 / Accepted: 10 November 2010 / Published: 12 November 2010 Abstract: Recently, KOD and its related DNA polymerases have been used for preparing various modified nucleic acids, including not only base-modified nucleic acids, but also sugar-modified ones, such as bridged/locked nucleic acid (BNA/LNA) which would be promising candidates for nucleic acid drugs. However, thus far, reasons for the effectiveness of KOD DNA polymerase for such purposes have not been clearly elucidated. Therefore, using mutated KOD