the biomechanics of amnion rupture an x-ray diffraction study羊膜破裂一个x射线衍射的生物力学研究.pdf

The Biomechanics of Amnion Rupture: An X-Ray Diffraction Study 1 2 3 4 5 4 4 Che J. Connon *, Takahiro Nakamura , Andy Hopkinson , Andrew Quantock , Naoto Yagi , James Doutch , Keith M. Meek 1 School of Pharmacy, University of Reading, Reading, United Kingdom, 2 Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan, 3 Department of Ophthalmology, Nottingham University, Nottingham, United Kingdom, 4 School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom, 5 Japan Synchrotron Radiation Research Institute, Hyogo, Japan Pre-term birth is the leading cause of perinatal and neonatal mortality, 40% of which are attributed to the pre-term premature rupture of amnion. Rupture of amnion is thought to be associated with a corresponding decrease in the extracellular collagen content and/or increase in collagenase activity. However, there is very little information concerning the detailed organisation of fibrillar collagen in amnion and how this might influence rupture. Here we identify a loss of lattice like arrangement in collagen organisation from areas near to the rupture site, and present a 9% increase in fibril spacing and a 50% decrease in fibrillar organisation using quantitative measurements gained by transmission electron microscopy and the novel application of synchrotron X-ray diffraction. These data provide an accurate insight into the biomechanical process of amnion rupture and highlight X-ray diffraction as a new and powerful tool in our understanding of this process. Citation: Connon CJ, Nakamura T, Hopkinson A, Quantock A, Yagi N, et al (2007) The Biomechanics of Amnion Rupture: An X-Ray Diffraction Study. PLoS ONE 2(11): e1147. doi:10.1371/journal.pone.0001147 INTRODUCTION