tenomodulin inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathytenomodulin抑制视网膜新生血管形成的小鼠模型oxygen-induced视网膜病变.pdf

Int. J. Mol. Sci. 2012, 13, 15373-15386; doi:10.3390/ijms131115373 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Tenomodulin Inhibits Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy Wei Wang 1,*, Zhongqiu Li 1, Tatsuhiko Sato 2 and Yusuke Oshima 2 1 Department of Ophthalmology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China; E-Mail: lizhqiu@ 2 Department of Ophthalmology and Visual Science, Osaka University Medical School, Yamadaoka, Suita, Osaka 5650871, Japan; E-Mails: tatsusatou@ (T.S.); oshima@ophthal.med.osaka-u.ac.jp (Y.O.) * Author to whom correspondence should be addressed; E-Mail: wendy_wen81@; Tel.: +86-10-8523-1343. Received: 14 September 2012; in revised form: 9 October 2012/ Accepted: 14 November 2012 / Published: 20 November 2012 Abstract: We aimed to determine the anti-angiogenic effect of tenomodulin (TeM) on retinal neovascularization in an oxygen-induced retinopathy (OIR) mouse model. OIR was induced in C57BL/6 mice by exposing seven-day-old mice to 75% oxygen for five days followed by room air for five days. Control mice were exposed to room air from birth until postnatal day 17. Mice received intravitreal injections of 1 μg of TeM in one eye and PBS in the contralateral eye at P7 before being exposed to 75% oxygen. Eyes were collected at postnatal day 17. Retinal blood vessel patterns were visualized