snake cathelicidin from bungarus fasciatus is a potent peptide antibiotics蛇抗菌肽从bungarus fasciatus是一个强有力的肽抗生素.pdf

Snake Cathelicidin from Bungarus fasciatus Is a Potent Peptide Antibiotics 1,4. 2,4. 3. 1,4 3 1,4 3 Yipeng Wang , Jing Hong , Xiuhong Liu , Hailong Yang , Rui Liu , Jing Wu , Aili Wang , Donghai Lin2*, Ren Lai1,3* 1 Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China, 2 Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China, 3 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu, China, 4 Graduate School of the Chinese Academy of Sciences, Beijing, China Abstract Background: Cathelicidins are a family of antimicrobial peptides acting as multifunctional effector molecules of innate immunity, which are firstly found in mammalians. Recently, several cathelicidins have also been found from chickens and fishes. No cathelicidins from other non-mammalian vertebrates have been reported. Principal Findings: In this work, a cathelicidin-like antimicrobial peptide named cathelicidin-BF has been purified from the snake venoms of Bungarus fasciatus and its cDNA sequence was cloned from the cDNA library, which confirm the presence of cathelicidin in reptiles. As other cathelicidins, the precursor of cathelicidin-BF has cathelin-like domain at the N terminus and carry the mature cathelicidin-BF at the C terminus, but it has an atypical acidic fragment insertion between the cathelin- like domain and the C-terminus. The acidic fragment is similar to acidic domains of amphibian antimicrobial precursors. Phylogenetic analys