枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定.PDF

枳壳及其制剂血府逐瘀胶囊中柚皮苷和新橙皮苷的含量测定  周燕，薛磊冰，金佩芬(嘉兴市食品药品检验检测院，浙江 嘉兴 314001) 摘要：目的 建立 HPLC 同时测定枳壳和血府逐瘀胶囊中柚皮苷和新橙皮苷的含量。方法 色谱柱为 SunFireTM C 18 1 (250 mm×4.6 mm ，5 μm) ；流动相为乙腈-水(20 ∶80)，磷酸调pH 至2.5 ；流速为1.0 mL·min ；检测波长为283 nm 。结 果 柚皮苷在0.166~2.074 μg 内线性关系良好(r=0.999 8) ，平均加样回收率为98.17% ；新橙皮苷在0.162~2.020 μg 内线性 关系良好(r=0.999 5) ，平均加样回收率为100.61%。结论 本方法简便、准确，重复性好，可用于枳壳及其制剂血府逐瘀 胶囊中柚皮苷和新橙皮苷的含量测定。 关键词：枳壳；血府逐瘀胶囊；柚皮苷；新橙皮苷；高效液相色谱法 中图分类号：R284.1 ；R917.101 文献标志码：B 文章编号：1007-7693(2016)06-0778-03 DOI: 10.13748/ki.issn1007-7693.2016.06.024 Determination of Naringin and Neohesperidin in Aurantii Fructus and its Preparation Xuefu Zhuyu Capsules ZHOU Yan, XUE Leibing, JIN Peifen(Jiaxing Drug and Food Vocational College, Jiaxiang 314001, China) ABSTRACT: OBJECTIVE To establish an HPLC method for the determination of naringin and neohesperidin in Aurantii Fructus and its preparation Xuefu Zhuyu Capsules. METHODS The column was SunFireTM C 18 (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(20 ∶80) adjusted to pH 2.5 with phosphoric acid. The flow rate was 1.0 mL·min1 and the detection wavelength was at 283 nm. RESULTS The calibration curves were linear within the range of 0.1662.074 μg (r=0.999 8) for naringin, 0.1622.020 μg(r=0.999 5) for neohesperidin, respectively. The average recovery of them were 98.17% and 100.61%, respectively. CONCLUSION The method is simple, practicable, accurate and rapid. It can be applied to content determination of naringin and neohesperidin in Aurantii Fructus and Xuefu Zhuyu Capsules. KEY WORDS: Aurantii Fructus; Xuefu Zhuyu Capsules; naringin; neohesperidin; HPLC 枳壳(Aurantii Fructus)是中国药典 2015 年版 别是判定其枳壳投料的正确性，参考有关文献[5-6] ， 收录的中药，药用来源为芸香科植物酸橙(Citrus