大鼠脑缺血后神经功能评分与脑梗死体积相关性分析.doc

研究报告 MicroRNA-424通过增加转录因子的表达减轻小鼠缺血性脑损伤 赵海苹王荣亮高志罗玉敏 (北京 100053) 摘要目的 研究对鼠缺血后。方法 μL）通过脑室注射，7 d后采用大脑中动脉线栓闭塞（MCAO）的方法建立鼠模型，动物分组：假手术组，假手术模型组，处理组（= 6）。缺血后组织，estern blot检测的。结果 可以鼠后Western blot结果显示，模型组织中蛋白表达。结论可通过鼠的表达，从而减轻缺血。 【关键词】 microRNA-424；脑缺血；【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2013.006.000 MicroRNA-424 increases the expression of transcriptional factors and reduces the ischemic brain injury in the mouse ZHAO Hai-ping, WANG Rong-liang, GAO Zhi, LUO Yu-min (Cerebrovascular Diseases Institute, Xuanwu Hospital, Capital Medical University, Beijing 100053, China) 【Abstract】 Objective To investigate the effect of microRNA-424 (miR-424) on neuronal apoptosis after cerebral ischemia in mice. Methods The lentiviral mediated overexpression of miR-424 (109 U/mL, 8 μL) was injected into the lateral ventricle in mice for 7 d, then the middle carotid artery occlusion (MCAO) model was established, and the mice were divided into 4 groups: sham group, sham + miR-424 group, MCAO model group and MCAO + miR-424 group (n = 6). At 8 h after MCAO, the ischemic brain tissue was removed and paraffin sections were TUNEL-stained to observe the neuronal apoptosis, and the protein levels of transcription factor PU.1, hypoxia inducible factor-1α (HIF-1α), apoptosis-related proteins p53 were detected by Western blot. Results The TUNEL immunofluorescence observation revealed that miR-424 reduced neuronal apoptosis at 8 h after cerebral ischemia in the mice. The Western blot showed that before ischemia and at 8 h after ischemia, miR-424 increased the levels of PU.1, HIF-1α and apoptotic protein p53 in the normal and MCAO model brain tissues, exhibiting the same effect on the expression of those three proteins. Conclusions miR-424 can reduce neuronal apoptosis after cerebral ischemia partly through increasing the expression of transcription factor PU.1 and HIF-1α, as well as apoptosis-related proteins p53 in the brai