antibiotics increase gut metabolism and antioxidant proteins and decrease acute phase response and necrotizing enterocolitis in preterm neonates抗生素增加肠道代谢和抗氧化蛋白质和减少急性期反应和早产新生儿坏死性小肠结肠炎.pdf

Antibiotics Increase Gut Metabolism and Antioxidant Proteins and Decrease Acute Phase Response and Necrotizing Enterocolitis in Preterm Neonates 1,3 3 3 3 Pingping Jiang , Michael Ladegaard Jensen , Malene Skovsted Cilieborg , Thomas Thymann , Jennifer 1 1 2 3 Man-Fan Wan , Wai-Hung Sit , George L. Tipoe , Per Torp Sangild * 1 School of Biological Sciences, The University of Hong Kong, Hong Kong, People’s Republic of China, 2 Department of Anatomy, The University of Hong Kong, Hong Kong, People’s Republic of China, 3 Department of Human Nutrition, University of Copenhagen, Frederiksberg, Denmark Abstract Background: The appropriate use of antibiotics for preterm infants, which are highly susceptible to develop necrotizing enterocolitis (NEC), is not clear. While antibiotic therapy is commonly used in neonates with NEC symptoms and sepsis, it remains unknown how antibiotics may affect the intestine and NEC sensitivity. We hypothesized that broad-spectrum antibiotics, given immediately after preterm birth, would reduce NEC sensitivity and support intestinal protective mechanisms. Methodology/Principal Findings: Preterm pigs were treated with antibiotics for 5 d (oral and systemic doses of gentamycin, ampicillin and metrodinazole; AB group) and compared with untreated pigs. Only the untreated pigs showed evidence of NEC lesions and reduced digestive function, as indicated by lowered villus height and activity of brush border enzymes. In addition, 53 intestinal and 22 plasma proteins differed in expression between AB and untreated pigs. AB treatme