体外培养视网膜M#252;ller细胞高压气体冲击损伤模型.pdf

·实验研究· 体外培养视网膜Miiller细胞高压气体冲击损伤模型 李传旭 谢伯林 尹志勇 冯 刚 周继宏 杨志焕 王正国 retinal A airwavetraumaticmodelinvitrocultured highpressure injury Mfillercell Li Gang，Zhou Zhihuan，WangZhengguo．Departmentof Chuanxu，XieBolin，YinZh咖ng，Feng Jihong，Yang General Ophthalmology，KunmingHospitalofChengduMilitaryCommand，Kunming650032，China Abstract Moilercellsarethe elementintheretinawhichtheentirethicknessofthe Objective predominant0ial span retina．When to cells andmolecular thereis study injuryretina，MOilerundergosignificantmorphological，cellularchanges．Present Moilercellandobservethe of and wastoestablishanew oftraumaticinculturedretinal pattern injury changesmorphology in retina． TheretinalMoilercellsof1-week·oldSDratswere and functionofretinalMOilercell damaged Methods separated culturedin 10％fetalbovine cellswereidentifiedGFAP seededin6-well and senlm．Passaged by plates DMEM／F12containing 7 additional cellsweredividedintotwo Mailercellsin staining．Afterdays incubation，the groupsrandomly：Culturedexperimental were toadevicewithdifferent of simulatetheMailercell direct air(25，50，100，150，200，250kPa)to by group joined pressure shock no