cortical neurovascular coupling driven by stimulation of channelrhodopsin-2受刺激的名为u201c大脑皮层神经与血管的耦合.pdf

Cortical Neurovascular Coupling Driven by Stimulation of Channelrhodopsin-2 1 2,3,4 2 2 1 1,2,3,4,5 Lijun Ji , Junli Zhou , Rabia Zafar , Svetlana Kantorovich , Ruixin Jiang , Paul R. Carney , Huabei Jiang1* 1The J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, United States of America, 2 Department of Pediatrics, University of Florida, Gainesville, Florida, United States of America, 3 Department of Neurology, University of Florida, Gainesville, Florida, United States of America, 4 McKnight Brain Institute, University of Florida, Gainesville, Florida, United States of America, 5 B. J. and Eve Wilder Center for Excellence in Epilepsy Research, University of Florida, Gainesville, Florida, United States of America Abstract While functional imaging is widely used in studies of the brain, how well the hemodynamic signal represents the underlying neural activity is still unclear. And there is a debate on whether hemodynamic signal is more tightly related to synaptic activity or action potentials. This study intends to address these questions by examining neurovascular coupling driven by pyramidal cells in the motor cortex of rats. Pyramidal cells in the motor cortex of rats were selectively transduced with the light sensitive cation channel channelrhodopsin-2 (ChR2). Electrophysiological recordings and optical intrinsic signal imaging were performed simultaneously and synchronously to capture the neural activity and hemodynamics induced by optical stimulation of ChR2-expressing pyramidal cells. Our results indicate that both synaptic activity (local field potential, LFP) and action potentials (m