新型香豆素类zn_2_荧光探针的合成及细胞成像研究_李长伟.pdf

DOI: 10.6023/cjoc201511016 研究论文 有机化学 Chinese Journal of Organic Chemistry ARTICLE 新型香豆素类Zn2＋荧光探针的合成及细胞成像研究 李长伟 杨 栋 尹 兵 郭 媛* (西北大学化学与材料科学学院 西安 710127) 摘要 设计、合成了一种光诱导电子转移(Photoinduced Electron Transfer, PET)型香豆素类水溶性 Zn2＋荧光探针 1 和 2. 通过荧光光谱分析实验发现, 该探针具有较好的选择性和灵敏度, 其荧光强度随着 Zn2＋的浓度增大而逐渐增强, 并可 成功实现对人乳腺癌细胞(MCF-7)和枯草杆菌(B. subtilis) 的标记. 目标探针分子结构均经 1H NMR, 13C NMR, IR, HRMS 及 X 单晶射线衍射进行了表征, 并且还获得了探针 2 与 Zn2＋络合物[Zn(2)] 的单晶结构, X 单晶衍射实验表明络 合物[Zn(2)] 中Zn2＋为五配位, 其几何构型为双锥体. 关键词 香豆素; Zn2＋荧光探针; 细胞成像 Novel Coumarin-Based Fluorescent Probes for Detecting Zn2＋ in Living Cells Li, Changwei Yang, Dong Yin, Bing Guo, Yuan* (College of Chemistry and Materials Science, Northwest University, Xian 710127) Abstract Zinc ion (Zn2＋) is one of the most important transition-metal ions in the human body which is involved in many important life activities and many diseases can be displayed by its situation. Thus, monitoring of Zn2＋ is very meaningful to diagnosis of diseases. Compared to the traditional detection methods, fluorescence probe is safer and more practical. Because coumarin derivatives possess several advantages in optics, we choose them as key structures to prepare new fluorescent probes. Based on the mechaism of photoinduced electron transfer (PET), the water-soluble fluorescent probes 1 and 2 that are couma- rin-based derivatives were designed, which demonstrated sensitivity for Zn2＋