zebrafish numb and numblike are involved in primitive erythrocyte differentiation斑马鱼麻木和numblike参与原始红细胞分化.pdf

Zebrafish Numb and Numblike Are Involved in Primitive Erythrocyte Differentiation 1 2 3 1 1 Erica Bresciani , Stefano Confalonieri , Solei Cermenati , Simona Cimbro , Efrem Foglia , Monica 3 2,4,5 . 1 . Beltrame , Pier Paolo Di Fiore * , Franco Cotelli * ` 1 Dipartimento di Biologia, Universita degli Studi di Milano, Milano, Italy, 2 The FIRC Institute for Molecular Oncology Foundation (IFOM) at the IFOM-IEO Campus, Milano, ` Italy, 3 Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy, 4 European Institute of Oncology (IEO), Milano, Italy, ` 5 Dipartimento di Medicina, Chirurgia ed Odontoiatria, Universita degli Studi di Milano, Milano, Italy Abstract Background: Notch signaling is an evolutionarily conserved regulatory circuitry implicated in cell fate determination in various developmental processes including hematopoietic stem cell self-renewal and differentiation of blood lineages. Known endogenous inhibitors of Notch activity are Numb-Nb and Numblike-Nbl, which play partially redundant functions in specifying and maintaining neuronal differentiation. Nb and Nbl are expressed in most tissues including embryonic and adult hematopoietic tissues in mice and humans, suggesting possible roles for these proteins in hematopoiesis. Methodology and Principal Findings: We employed zebrafish to investigate the possible functional role of Numb and Numblike during hematopoiesis, as thi