whole genome sequencing of mutation accumulation lines reveals a low mutation rate in the social amoeba dictyostelium discoideum全基因组测序的突变积累行揭示了一个社会变形虫dictyostelium discoideum突变率较低.pdf

Whole Genome Sequencing of Mutation Accumulation Lines Reveals a Low Mutation Rate in the Social Amoeba Dictyostelium discoideum 1 ¤ 1,2 1 2 2 2 Gerda Saxer * , Paul Havlak , Sara A. Fox , Michael A. Quance , Sharu Gupta , Yuriy Fofanov , Joan E. Strassmann3, David C. Queller3 1 Department of Ecology and Evolutionary Biology, Rice University, Houston, Texas, United States of America, 2 Center for Biomedical and Environmental Genomics, University of Houston, Houston, Texas, United States of America, 3 Department of Biology, Washington University in St. Louis, St. Louis, Missouri, United States of America Abstract Spontaneous mutations play a central role in evolution. Despite their importance, mutation rates are some of the most elusive parameters to measure in evolutionary biology. The combination of mutation accumulation (MA) experiments and whole-genome sequencing now makes it possible to estimate mutation rates by directly observing new mutations at the molecular level across the whole genome. We performed an MA experiment with the social amoeba Dictyostelium discoideum and sequenced the genomes of three randomly chosen lines using high-throughput sequencing to estimate the spontaneous mutation rate in this model organism. The mitochondrial mutation rate of 6.76 61029, with a Poisson confidence interval of 4.1 61029 2 9.5 61029, per nucleotide per generation is slightly lower than estimates for other taxa. The mutation rate estimate for the nuclear DNA of 2.9 610211, with a Poisson confidence interval ranging from 7.4 610213 to 1.6610210, is the lowest reported for any eukaryote. These results are consistent w