最新复方扶芳藤合剂对大鼠骨髓间充质干细胞增殖的影响及其机理的探讨.pdf

方扶芳藤合剂含药血清促进 BMSCs 增值过程中，复方扶芳藤合剂含药 血清可以使Notch1 、Jagged1 、hes1 的mRNA 和蛋白表达上调；(3)复方 扶芳藤合剂通过激活Notch 信号通路对BMSCs 促进增殖，复方扶芳藤合 剂对Notch 信号通路的影响可能是抗衰老机制之一。 关键词：复方扶芳藤合剂；大鼠骨髓间充质干细胞；增殖；NOTCH 信 号通路 3 Effect of Compound Fufangteng Mixture of rat bone marrow mesenchymal stem cells on proliferation and the possible mechanism ABSTRACT Objective: To establish a culture system of rat bone mesenchymal stem cells by Cell Culture Technique in vitro. According to observing the regulation of Compond Fufangteng Mixture durg-containing serum on Notch signa pathway during MSCs proliferation, to clarify the mechanism of MSCs proliferation promoted by Compond Fufangteng Mixture , and to explore a new way of anti-aging for Chinese medicine and fomulas. Methods : (1) Used Cell Culture Technique in vitro to establish rat bone mesenchymal stem cells culture system and observed the cell morphological characteristics at different stages ;(2) Cell growth curves were drawn by MTT method; The cell cycle and expression of BMSCs surface antigens were detected by flow cytometer; BMSCs were induced to differentiate into osteoblasts. Through the above method to identify BMSCs;(3) A total of 30 rats were randomly divided into 2 groups, 15 per group, and Compound Fufangteng Mixture was given by gavage for the rats as drug intervention group,while physiological saline was given as negative control group. Use the 10% medicated serum and negative control to intervene BMSCs. Then used MTT method to test the effect of Compound Fufangteng Mixture