人恶性胶质瘤干细胞的分离与鉴定.pdf

72 , 实验研究 1 2 1 1 1 1 李光辉 , 王东林 , 陈正堂 , 尹昌林 , 纪华 , 吕胜青 1. 第三军医大学新桥医院( 全军肿瘤研究所:李光辉, 陈正堂,纪华; 神经外科: 尹昌林, 吕 青) , 重庆400037 2. 同济大学附属同济医院肿瘤科, 上海200065 Isolation and identification of cancer stem cells in humangliomas 1 2 1 1 1 1 LI Guang-hui , WA N G D ong-lin , CH EN Zheng-tang , YIN Chang-lin , J I H ua , L U S heng-qing 1. X inqiao H os ital , Third M ilitary Medical University, Chongqing 400037, P . R. China 2. De artment of Oncology, Tongj i H os ital of Tongj i University , S hanghai 200065, P . R. China : 从人恶性胶质瘤中分离并鉴 [ ABSTRACT] OBJECTIVE : T o isolate and ident ificate the brain 定胶质瘤干细 : 采用神经干细 无 tumor stem cells ( BTSCs) within human gliomas tissue in v itro . 血清培养方法, 分离胶质瘤干细 , 免疫细 METHODS: By switching primary human glioblastoma cells int o a 化学法有限梯度稀释实验二代球体形成分 def ined serum-free neural stem cells medium, glioblastoma 析流式细 分析等方法对胶质瘤干细 进 spheres were isolated. By limiting dilution assay, secondary 行鉴定: 3 例患者原代培养胶质瘤细 spheres forming assay, flow cytomet ry assay and so on, BTSCs + 中 CD133 细 分别为 74% 28% 和 w ere ident ificated. RESULTS: Glioblastoma spheres consisted of 42% 每 100 个原代胶质瘤细 中可形成 BT SCs expressing CD133 and nestin but no GFAP and M AP2, + 1 个左右的胶质瘤细 球; 胶质瘤细 球细 and a few BTSCs expressed MBP . The percentage of CD133 cell 均表达神经干细 标