陆春风：双氢青蒿素通过激活Nrf促进肝星状细胞坏死性凋亡的研究(英文).doc

————————————————————————————————————————————————————— 陆春风：双氢青蒿素通过激活Nrf2促进肝星状细胞坏死性凋亡的研究(英文) 中华中医药杂志(原中国医药学报)2016年5月第31卷第5期 CJTCMP,May 2016,Vol.31,No.5 [25] 李建生,王至婉,余学庆,等.支气管哮喘不同分期证素演变规 律研究.中医杂志,2015,56(13):1131-1135 [26] 陈博宇.糖尿病肾病5期血液透析前后证素演变规律的研究. 北京:北京中医药大学,2009 [27] 张伯礼,康立源,项耀祖.中药新药临床试验中有关疗效评价 若干问题的思考.中国新药与临床杂志,2007,26(11):861-863 [28] 李四维,刘洋,徐浩.中医证候演变规律研究方法的现状与展望. 中国中西医结合杂志,2011,31(6):854-857 [29] 孙晓伟,王阶.证候动态变化规律研究.山东中医药大学学报, 2010,34(2):104-105? 1919 ?[30] 季聪华,曹毅,陈健,等.证型变化随机对照试验的盲法技术. 中国中西医结合杂志,2014,34(7):869-872[31] 赵晨,卞兆祥,商洪才.辨证论治基本原则下中医群体化研究的思考及二维RCT设计方法的提出.中华中医药杂志,2015, 30(5):1391-1396[32] 田峰,谢雁鸣.真实世界研究:中医干预措施效果评价的新理念. 中西医结合学报,2010,8(4):301-306（收稿日期：2015年11月28日） · Treatise · Study of Dihydroartemisinin on inducing necroptosis through activating Nrf2 in hepatic stellate cells in vitro LU Chun-feng1, XU Wen-xuan1, ZHANG Feng1,2,3, ZHENG Shi-zhong1,2,3 ( 1School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; 2Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China; 3The National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing 210023, China ) stellate cells (HSCs). Methods: HSCs were divided into control cells and DHA-treated cells. 3-(4, 5-dimethylthiazol-2-Abstract: Objective: To investigate the in vitro effect of Dihydroartemisinin (DHA) on necroptosis in hepatic yl)-2, 5-diphenyltetrazolium bromide (MTT), cell counting kit-8 (CCK-8), and methylene blue staining were used to examine cell viability. Enzyme-linked immunosorbent assay (ELISA) was used to detect high-mobility group box 1 (HMGB1) level in culture medium. Real-time PCR was performed to detect HMGB1 mRNA level. Cell Titer-Glo kit was used to detect intracellular adenosine triphosphate (ATP) level. Lactate dehydrogenase (LDH) kit