白藜芦醇抑制大鼠海马CA1区神经元放电.pdf

Acta Physiologica Sinica , June 25, 2005, 57 (3): 355-360 355 Research Paper Resveratrol inhibits neuronal discharges in rat hippocampal CA1 area 1 1,* 2 1 1 1 LI Ming , WANG Qing-Shan , CHEN Yi , WANG Ze-Min , LIU Zheng , GUO Shu-Mei 1Department of Physiology, Institute of Basic Medicine; 2Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China Abstract: The effects of resveratrol on the discharges of neurons in CA1 area of rat hippocampal slices were examined by using extracellular recording technique. The results are as follows: (1) In response to the application of resveratrol (0.05, 0.5, 5.0 µmol/L, n=52) into the superfusate for 2 min, the spontaneous discharge rate of 46/52 (88.5%) neurons was significantly decreased in a dose- dependent manner; (2) Application of L -glutamate (0.2 mmol/L) into the superfusate led to a marked increase in discharge rate of all 8 (100%) slices in an epileptiform pattern. The increased discharges were suppressed by application of resveratrol (5.0 µmol/L); (3) In 7 slices, perfusion of the selective L-type calcium channel agonist, Bay K8644 (0.1 µmol/L), induced a significant increase in the discharge rate of 6/7 (85.7%) slices. The increased discharges were suppressed by application of resveratrol (5.0 µmol/L); (4) In 9 slices, perfusion of nitric oxide synthase (NOS) inhibitor NG-nitro-L -arginine methyl ester (L -NAME, 50 µmol/L) into the superfusate significantly augmented the discharge rate of 7/9 (77.8%) slices. Resveratrol (5.0 µmol/L) applied into the superfusate reduced the increased discharges of all 7/7 (100%) neurons; (5) In 10 units, the large-conductance Ca2+-activated K+ channel blocker (tetraethylammonium chloride, TEA, 1 mmol/L) significantly increased the discharge rate of 9/10 (90%) slices. Resveratrol (5.0