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Identification of Differentially Expressed Genes in Breast Tumors From African American Compared With Caucasian Women英文版.pdf

发布:2018-09-26约6.4万字共11页下载文档
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Original Article Identification of Differentially Expressed Genes in Breast Tumors From African American Compared With Caucasian Women 1 2 1 3 3 Lori A. Field, PhD ; Brad Love, PhD ; Brenda Deyarmin, HT ; Jeffrey A. Hooke, MD ; Craig D. Shriver, MD ; and Rachel E. Ellsworth, PhD4 BACKGROUND: Breast tumors from African American women have less favorable pathological characteristics and higher mortality rates than those of Caucasian women. Although socioeconomic status may influence prognosis, bio- logical factors are also likely to contribute to tumor behavior. METHODS: Patients with invasive breast cancer were matched by age, grade, and estrogen receptor status; patients with benign disease were matched by age and diag- nosis type. RNA from laser microdissected tumors and whole-sectioned nonmalignant breast tissues was hybridized to HG U133A 2.0 microarrays. Data were analyzed using Partek Genomics Suite using a cutoff of P .001, 1.5-fold change, and results were validated by quantitative real-time polymerase chain reaction. RESULTS: Clinicopathological factors did not differ significantly between groups for age at diagnosis, tumor size or stage, lymph node or human epidermal growth receptor 2 status, intrinsic subtype, or mortality. Two-way analysis of the tumor specimens revealed 25 probes representing 23 genes differentially expressed between populations; hierarchical clustering classified 24 of 26 African American women and 25 of 26 Caucasian women correctly. In the nonmalignant specimens, 15 probes rep- resenting 13 genes were differentially expressed, including 5 genes that also differed in the tumor specimens; these genes were able to correctly classify nonmalignant breast
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