考马斯亮蓝显色剂变色反应机理的研究.pdf

2002 60 Vol. 60, 2002 9 , 1656~ 1661 ACTA CHIMICA SINICA No. 9, 1656~ 1661 a, b X , a a a 曹稳根 焦庆才 刘 茜 陈 雷 a ( 210093) b ( 234000) G-250 ( CBBG) , CBBG . , CBBG y y , CBBG . , , , Study on the Mechanism of Color Changes of Commassie Brilliant Blue G-250 a, b X , a a a CAO, Wen-Gen IAO, Qing-Cai LIU, Qian CHEN, Lei a ( Pharmaceutic Biotechnology Key Laboratory, College of Lfie Science , Nanj ing University, Nanj ing 2 0093) b ( Dep artment of Biochemistry, Suzhou Teachers. College, Suzhou 234000) Abstract The mechanism of color changes of Commassie Brilliant Blue G-250 ( CBBG) has been investigated by spectrometry. The influence of different experimental conditions on the absorption spectra of CBBG was inspected. It is found that CBBG forms aggregate by hydrophobic interaction in high phosphoric acid concentration. Due to the difference in the aggregative degree, CBBG shows color changes from blue to green to brown in the system. Keywords Commassie Brilliant Blue G-250, mechanism of color changes, spectrometry, hydrophobic interaction , . , , [ 8, 9] . , , . 197