课件3核苷酸代谢.PPT
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* 10 5-甲酰胺基-4-甲酰胺咪唑核苷酸 次黄嘌呤一磷酸 环化酶 * Second, Making AMP and GMP * kinase ADP kinase GDP ATP GTP GDP AMP GTP kinase GDP kinase ADP GTP ATP ADP GMP ATP ADP, ATP, GDP and GTP biosynthesis * Regulation of De Novo Synthesis of Purine Nucleotides Gln:PRPP amidotransferase Ribose-5-phosphate PRPP 5-Phosphoribosylamine IMP XMP AMP ADP ATP GMP GDP GTP Adenylosuccinate + Adenylosuccinate Synthesase Ribose-5-phosphate Pyrophosphokinase IMP dehydrogenase 2 Active Inactive Key Step * Interconversion of Purine nucleotides XMP AMPS (腺苷一磷酸代琥珀酸) IMP NH3 Adenine Deaminase NADP++ NH3 NADPH Guanine Reductase AMP GMP * Deoxyribonucleotide Biosynthesis NADPH Adenine Guanine Cytosine Uracil Phosphorylase dNMP dNTP Kinase DNA Thymine × dTTP ? * ATP 腺嘌呤核苷 谷氨酰胺, H2O 嘌呤核苷酸的生物合成 5-磷酸核糖 5-磷酸核糖-1-焦磷酸 5-磷酸核糖胺 IMP 次黄嘌呤 XMP 黄嘌呤 dGMP dGDP dGTP GMP GDP GTP AMP ADP ATP dAMP dADP dATP 腺嘌呤 鸟嘌呤 谷氨酸, PPi AMP * dTMP dTDP dTTP Deoxythymidine ATP ADP Deoxythymidine kinase dUMP dUDP dCDP N5,N10-methylene-tetrahydrofolic Acid ATP ATP ADP ADP 脱氧胸腺嘧啶核苷 dUTP dCMP 脱氧胞嘧啶核苷 胞嘧啶核苷 CMP CDP 尿嘧啶核苷 CTP UMP UDP UTP dCTP 谷氨酰胺 + CO2 天冬氨酸 嘧啶核苷酸的生物合成 H2O ATP 氨甲酰磷酸 * Ribonucleotides can be converted to deoxyribonucleotides by ribonucleotide reductase The (-S-S-)/(-SH HS-) oxidation-reduction cycle involving ribonucleotide reductase, thioredoxin, thioredoxin reductase and NADPH. 58-kD 12-kD 86 kD?2 43.5 kD ?2 硫氧还蛋白还原酶 硫氧还蛋白 核苷酸还原酶 * GSSG 2GSH NDP Reductase Glutaredoxin 2SH GSSG * Ribonucleotide reductase Allosteric regulation 225 462 底物特异性调节位点 酶活调节位点 底物结合位点 * The free radical mechanism of ribonucleotide reduction ? unit ? unit substrate ? ? Fe3+ ? ? Fe2+ ? ? ? * 1 mnqnllvtkr dgsterinld kihrvldwaa eglhnvsisq velrshiqfy dgiktsdihe 61 tiikaaadli srdapdyqyl aarlaifhlr kkaygqfepp alydhvvkmv emgkydnhll 121 edyteeefkq mdtfidhdrd mtfsyaavkq legkylvqnr vtgeiyesaq flyilvaacl 181 fsnypretrl qyvkrfydav stfkislptp imsgvrtptr qfsscvliec gdsldsinat 241 ssaivkyvsq ragiginagr iralgspirg geafhtgcip
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