SiRNA 干扰Prohibitin基因表达对人前列腺癌PC-3细胞生长及凋亡影响.doc

  SiRNA 干扰 Prohibitin 基因表达对人前列腺 癌 PC-3 细胞生长及凋亡影响# 1,2 1 1 1  5 10 15 20 25 30  （1. 华南肿瘤学国家重点实验室,中山大学肿瘤防治中心泌尿外科, 广东 广州 510060； 2. 广东省泌尿外科重点实验室 (2010A060801016),广东 广州 510060） 摘要： 目的：观察 RNAi 抑制前列腺癌 PC-3 细胞中的 PHB 表达对细胞增殖、凋亡影响，探 讨 PHB 对人前列腺癌的可能作用机制。方法：SiRNA 基因沉默 PC-3 细胞中 PHB 基因，Annexin V/PI 双染和流式细胞计数观察 NM 和 CDM 培养下及分别给予血小板源性生长因子 PDGF 或 EGF 刺激的前列腺癌凋亡细胞数目差别 。结果：流式细胞仪(FCM)检测发现，在 siRNA 抑制 PC-3 细胞中的 PHB 后，CDM 实验组细胞凋亡率显著增加，同 NM 对照组比较有显著性差异(p 0.05 )；在 CDM 组给予 PDGF 或 EGF 后，细胞凋亡率升高，与 NM 对照组相比较有显著性差 异(p 0.05 )。结论：低胆固醇培养条件下前列腺癌细胞生长抑制，给予生长刺激因子促进 细胞凋亡，低胆固醇抑制前列腺癌 PC-3 细胞体外增殖活性可能同 PHB 表达升高有关。 关键词：Prohibitin，RNAi，前列腺癌细胞，胆固醇，细胞凋亡 中图分类号：请查阅《中国图书馆分类法》 Effects of SiRNA prohibitin expression on proliferation and apoptosis in human prostate carcinoma PC-3 cells. dongpei1,2, jianglijuan1, guoshengjie1, zhoufangjian1 (1. State Key Laboratory of Oncology in Southern China, Department of Urology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, China; 2. Guangdong Key Laboratory of Urology，The first affiliated Hospital of Guangzhou medical University, Guangzhou, Guangdong, 510060) Abstract: Objective:To observe the effects of RNAi inhibit PHB on cell growth,and apoptosis in Pc-3 cells.Explore the possible molecular mechanism of PHB on human prostate cancer.Methods:SiRNA knockdown PHB in PC-3 cells and treated cells with NM or CDM added/or not PDGF /EGF. Apoptosis was determined by Annexin V/PI double staining technique and flow cytometry.Results： Annexin V/PI double staining and flow cytometry discovered that after using siRNA knockdowned PHB in PC-3 cells,PC-3 cells apoptosis rate of cultured in CDM was significantly higher than cells cultured in NM(p0.05).Addred PDGF or EGF into CDM can upon apoptosis rate(p0.05 ).Conclusions:Cholesterol depleted conditions can induce apoptosis in PC-3 cells. Cholesterol depleted conditions add growth factors can promote apoptosis in PC-3 cells. Key words: Prohibitin; RNAi；Prostate carcinoma cells; Cholesterol;A