甘蓝和白菜紫色酸性磷酸酶17基因家族的克隆和比较分析.pdf

作物学报 ACTA AGRONOMICA SINICA 2010, 36(3): 517525 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.00517 甘蓝和白菜紫色酸性磷酸酶17 基因家族的克隆和比较分析 卢 坤1,2 张 凯1,2,** 柴友荣1,2 陆俊杏1,2 唐章林 1,2 李加纳1,2,* 1 , 400716; 2 , 400716 : 2 PAP17 , , PAP17 I (BoPAP17-1 BrPAP17-1) II (BoPAP17-2 BrPAP17-2) Southern , 2 PAP17 , , PAP17 , PAP PCR , PAP17 9 , , , , , BrPAP17-2 , BrPAP17 BoPAP17 , , 4 d 8 d ; 4 d , , PAP17 PAP17 : ; ; ; ; Cloning and Comparative Analysis of PURPLE ACID PHOSPHATASE 17 Gene Families in Brassica oleracea and Brassica rapa LU Kun1,2, ZHANG Kai1,2,**, CHAI You-Rong1,2, LU Jun-Xing1,2, TANG Zhang-Lin1,2, and LI Jia-Na1,2,* 1 College of Agronomy and Life Sciences, Southwest University, Chongqing 400716, China; 2 Chongqing Rapeseed Technology Research Center, Chongqing 400716, China Abstract: Two PAP17 genes were cloned from parent species of Brassica napus, B. oleracea, and B. rapa, respectively. Accord- ing to sequence similarity, PAP17 genes could be divided into two types, type I (BoPAP17-1 and BrPAP17-1) and type II (BoPAP17-2 and BrPAP17-2). Southern hybridization resulted in two bands both in B. oleracea and B. rapa, this is accordance with former cloning results. Phytogenetic and molecular evolut