多波长RP_HPLC法测定双黄连口服液中黄芩苷_绿原酸和连翘苷_杜英峰.pdf

2009 9 中成 药 Septem ber 2009 31 9 Ch inese Trad itional PatentM ed icine Vol. 31 No. 9 [ ] RP-HPLC 1 1* 2 1 1 1 杜英峰 , 张兰桐 , 靳怡然, 许慧君, 刘静荣, 张立段 ( 1. 河北医科大学药学院药物分析教研室, 河北 家庄 050017; 2. 河北医科大学第二医院, 河北 家庄 050000) : H PLC; ; ; ; : : () : RP-HPLC, Agilent Zorbax SB C ( 250 mm @ 4. 6 mm, 5 Lm ), : ( A) 0. 3% ( B) 18 ; 14% ~ 20% /0~ 12 m in, 20% ~ 30% / 12~ 13 m in, 30% ~ 65% / 13~ 25 m in 65% /25~ 35 m in; : 30 e ; : 1. 0 mL /m in: 0. 225~ 3. 60 L g, 0. 032 4~ 0. 518 4 L g, 0. 135 ~ 2. 16 L g, 100. 5 % , 102. 0% , 99. 62 % , R SD 0. 75% , 3. 9% , 1. 1% : , : R927. 2 : A : 1001-1528( 2009) 09-1368-04 Simultaneousdetermination ofbaicalin, chlorogenic acid and phillyrin in Shuang huanglianOralLiquid bymultiplewavelengthRP-HPLC 1 2 1 1 DU Y ing-feng, ZHANG Lan-tong, JIN Y -i ran , XU H i-j n , LIU Jing-rong, ZHANG L-i d an (1. D epa rtm en t of Pharmaceutical A naly sis, S ch oo l of Pharmacy, H ebe iM ed ical Un iv ers ity, S hi iaz huang 050017, C hina; 2. Th e S econd H osp i ta l of H ebei M ed ica l Un iv ers ity, Sh i iaz huang, 050000, China ) EYWORDS: HPLC; Sh angh anglian O ralL iq id; baicalin; chlorogen ic acid; phillyrin ABSTRACT: AIM: To establish amethod for smi ltaneo sly determ ining baicalin, chlorogen ic acid and phillyrin in Sh angh anglian O ralL iq id(R ad ix S cutellariae, F los L on icerae ap on icac, F ructus F orsy th iae ). METHODS: A m ltip le w avelength HPLC method w as devoloped. The analysis w as performed on an Agilent Zorbax SB C18 col mn ( 250 mm @ 4. 6mm, 5 Lm ) w ith linear gradient el tion