多糖论文迭鞘石斛所含多糖对小鼠免疫细胞影响的研究.doc

多糖论文：迭鞘石斛所含多糖对小鼠免疫细胞影响的研究 【中文摘要】石斛是我国常用的贵重药材,它具有益胃生津、滋阴清热、润肺止咳等功效。现代药理学研究发现,石斛不但具有治疗白内障、降血糖、增强人体免疫力的功能,还具有抗肿瘤、抗氧化、抗衰老和抗血栓等作用。石斛多糖作为石斛属植物重要的药用成分之一,已被证实具有增强免疫力的作用。为进一步探讨和研究石斛多糖对免疫力提高的作用机制,本研究采用正常小鼠免疫细胞体外培养的方法,通过添加提取自迭鞘石斛(Dendrobinm chryseum Rolfe.)的多糖成分,并给予LPS和ConA刺激,观察这类多糖对小鼠脾细胞和小鼠腹腔巨噬细胞增殖作用的影响以及对细胞因子的影响。(1)研究迭鞘石斛所含多糖对小鼠脾淋巴细胞以及腹腔巨噬细胞增殖的影响；(2)研究迭鞘石斛所含多糖对小鼠细胞因子的影响。方法：(1)分离提取小鼠脾淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以MTT法检测这些免疫细胞增殖的变化情况。(2)分离提取小鼠脾淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以ELISA法检测培养液中所含IL-2、IL-6的含量。结果：(1)迭鞘石斛所含多糖在体外对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的增殖有增强作用,说明对小鼠具有免疫增强作用。(2)通过迭鞘石斛多糖对小鼠细胞因子影响的实验发现,迭鞘石斛多糖对小鼠脾淋巴细胞在ConA刺激下分泌IL-2具有明显促进作用(p0.01),并且具有量效关系；对小鼠腹腔巨噬细胞在LPS刺激下分泌IL-6也具有明显的促进作用(p0.01),并且具有量效关系。结论：(1)通过迭鞘石斛多糖对小鼠免疫细胞增殖的实验发现,迭鞘石斛多糖对于小鼠脾淋巴细胞以及腹腔巨噬细胞都有一定的促进作用,低浓度时,作用不显著(2)通过迭鞘石斛多糖对小鼠细胞因子影响的实验发现,迭鞘石斛多糖对小鼠脾淋巴细胞在ConA刺激下分泌IL-2具有促进作用,并且具有量效关系；对小鼠腹腔巨噬细胞在LPS刺激下分泌IL-6也具有促进作用,并且具有量效关系。 【英文摘要】Dendrobium as an expensive medicinal herb, which has many important effects, has been widely used in China. Modern pharmacology study showed that Dendrobium has many therapeutic effects, such as improving cataract, antitumor, enhancing immunity, reducing blood sugar, antioxidation, antiaging and antithrombotic. Polysaccharides from Dendrobinm as one kind of important effective components in Dendrobinm has been proven to be able to enhance immunity. In order to further study the action mechanism of Dendrobinm polysaccharides on immunity, this study investigated the effects of polysaccharides from Dendrobinm chryseum Rolfe on growthing of mice spleen cells and peritoneal macrophages stimulated by LPS and ConA with MTT, and on expression of some cytokines of the cells with ELISA.:(1) To study the effects of the polysaccharides extracted from Dendrobinm chryseum Rolfe on proliferation of mice spleen lymphocytes and peritoneal macrophages. (2) To research the influences of the polysaccharides extracted from Dendrobinm chryseum Rolfe on IL-2, IL-6