绿茶多酚对小鼠移植性T细胞免疫的影响.pdf

Clinical Immunology 110 (2004) 100– 108 /locate/yclim Effects of green tea polyphenols on murine transplant-reactive T cell immunity Jo¨rg Bayer, Alla Gomer, Yilmaz Demir, Hiroyuki Amano, Danielle D. Kish, Robert Fairchild, and Peter S. Heeger * The Transplantation Research Program, The Department of Immunology and The Glickman Urologic Institute, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA Received 11 August 2003; accepted with revision 20 October 2003 Abstract Green tea polyphenols (GrTP), the active ingredient of green tea, may have immunosuppressive properties, but whether and how GrTP affect transplant-reactive T cells is unknown. To address this, we tested the effects of GrTP on in vitro and in vivo transplant-reactive T cell immunity. GrTP inhibited IFNg secretion by cultured monoclonal T cells and by alloreactive T cells in mixed lymphocyte reactions. Oral GrTP significantly prolonged minor antigen-disparate skin graft survival and decreased the frequency of donor-reactive interferon gamma- producing T cells in recipient secondary lymphoid organs compared to controls. In contrast to other hypothesized actions, oral GrTP did not alter dendritic cell trafficking to lymph nodes or affect metalloproteinase activity in the graft. This is the first report of an immunosuppressive effect of GrTP on transplant-reactive T cell immunity. The results suggest that oral intake of green tea could act as an adjunctive therapy for prevention of transplant rejection in humans. D 2003 Elsevier Inc. All rights reserved. Keywords: T