文献_Competitive Amplification of Differentially Melting.pdf

METHODS OFFICIAL JOURNAL Competitive Amplification of Differentially Melting Amplicons (CADMA) Enables Sensitive and Direct Detection of All Mutation Types by High-Resolution Melting Analysis Lasse S. Kristensen,1∗ Gitte B. Andersen,1 Henrik Hager,2 and Lise Lotte Hansen1 1Department of Biomedicine, Aarhus University, Denmark; 2 Department of Pathology, Aarhus University Hospital, Denmark Communicated by Mats Nilsson Received 10 February 2011; accepted revised manuscript 16 August 2011. Published online 7 September 2011 in Wiley Online Library (/humanmutation).DOI: 10.1002/humu.21598 Introduction ABSTRACT: Sensitive and specific mutation detection is of As the fields of cancer diagnostics and treatment move toward the particular importance in cancer diagnostics, prognostics, era of personalized medicine, the identification of molecular mark- and individualized patient treatment. However, the major- ers becomes increasingly important. Several well-characterized mu- ity of molecular methodologies that have been developed tations have already been implemented in the clinics as markers of with the aim of increasing the sensitivity of mutation test- response to different t