HPLC同时测定藏药圆柏和刺柏中3种黄酮的含量.doc

HPLC同时测定藏药圆柏和刺柏中3种黄酮的含量 武雪1，宋平顺2，赵建邦3 （1.兰州大学药学院2012级硕士研究生，兰州730000；2. 3.甘肃省药品检验研究院，甘肃省中药品质与安全评价工程技术研究中心，兰州730070) [摘要] 目的：建立了HPLC法测定藏药圆柏和刺柏中3种主要黄酮成分的方法。方法：采用HPLC，样品经70%甲醇超声，采用色谱柱C18（4.6mm×250mm，5μm），0.2%磷酸乙腈-0.2%磷酸水为流动相进行液相色谱梯度洗脱，流速1.0ml·min-1，检测波长360nm，柱温：40℃。结果：进样量芦丁在0.0049～0.1464μg（R2 =0.9998）；槲皮苷在0.0094～0.2814μg（R2 =0.9997）；山奈酚在0.0054～0.1620μg（R2 =0.9996）均成良好的线性关系；芦丁、槲皮苷和山奈酚的加样回收率分别为100.12%（RSD2.55%），96.35%（RSD0.72%），96.13%（RSD0.49%）（n=6）。结论：3种黄酮在40min内达到基线分离。该方法分析时间短、稳定性和准确度良好，对圆柏和刺柏药材的质量控制具有一定的参考价值。 [关键词] 藏药； 圆柏；刺柏；高效液相色谱；黄酮；芦丁；槲皮苷；山奈酚 Determination of Three Main flavonoids in??Sabina chinensis and Juniperus formosana by HPLC Wu Xue1 ，Song Ping-Shun2，Zhao Jian-Bang3，（1.2012 Master in Reading，College of Pharmacy in Lanzhou University，Lanzhou73000；2，3．Gansu Institute for Food and Drug Control，Gansu Traditional Chinese Medicine Quality and Safety Evaluation Engineering Research Center， Lanzhou 73000) [Abstract] Objective：A HPLC method has been established for determination of main flavonoids in Sabina chinensis and Junipeius formosana. Method：Samples were extracted in 70% methanol with ultrasonic process. The HPLC was performed on an ODS-C18 column(4.6mm×150mm,5μm）with 0.2% H3PO4acetonitrile-0.2% H3PO4 as mobile phase. The flow rate was 1.0ml·min-1and the column temperature was kept at 40℃. The detective wavelength was 360 nm. Result：The linear range of rutin was 0.0049～0.1464μg（R2=0.9998）; the linear rang of quercetin-3-rhamnoside was 0.0094～0.2814μg（R2=0.9997）and the linear range of kaempferol was 0.0054～0.1620μg（R2=0.9996）. The average recovery of rutin; quercetin-3-rhamnoside and kaempferol were 100.%（RSD%），%（RSD%），（n=6Conclusion：The results showed that three Flavonoids reached baseline separation within 40min. This method has advantages of using little analytic time, a good stability and accuracy of operation, has some references value for establishing standardization quality control method of Sabina chinensis and Junipeius formosana herb. [Key Words] S