纳米材料和蛋白相互作用.pdf

Research Article Influence of the Surface Functional Group Density on the Carbon- Nanotube-Induced α‑Chymotrypsin Structure and Activity Alterations Xingchen Zhao,† Fang Hao,† Dawei Lu,† Wei Liu,‡ Qunfang Zhou,*,† and Guibin Jiang† †State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P. R. China g ‡Institute of Chemical Safety, Chinese Academy of Inspection and Quarantine, Beijing 100124, P. R. China r o . s c S a. *Supporting Information s b u p / / : ABSTRACT: Because of the special properties of carbon p t t h nanotubes (CNTs), their applications have been introduced to | 5 5 9 many fields. The biosafety of these emerging materials is of 1 8 0 5 2, 0 high concern concomitantly. Because CNTs may initially bind b 5 5 1 i. with proteins in biofluids before they exert biological effects, it r m e b a is of great importance to understand how the target proteins s m c e a interact with these exogenous nanomaterials. Here we / t 1 p e 2 investigated the interaction between α-chymotrypsin (α- S 0 1 n 0. ChT) and carboxylized multiwalled CNTs in a simulated o 1 S : biophysical environment utilizing the techniques of fluores- E i o E