应激对同型半胱氨酸代谢的负性调节.pdf

Acta Physiologica Sinica, August 25, 2004, 56 (4): 521-524 521 : Acta Physiologica Sinica, June 25, 2004, 56 (3): 269-274 * , 300050 : , (homocysteine, HCY) , , - HCY , β (cystathionine beta-synthase, CBS) , RT-PCR Northern blot CBS mRNA , ; CBS , , ; CBS mRNA (P0.05), 3 70.6% 5.9% 55.9% 4.3% , HCY , CBS ; HCY : ; ; ; β : Q418 Negative regulation of homocysteine metabolism by stress in rats * WU Shu-Qing, QIAN Ling-Jia Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050 , China Abstract : To investigate the effect of stress on homocysteine metabolism in the rat and explore the mechanism as well as the key regulatory link of stress-induced hyperhomocysteinemia, male Wistar rats were treated with restraint stress while control rats received routine treatment. By HPLC-fluorometry, the homocysteine level in rat plasma was determined. Cystathionine beta-synthase (CBS) activity in blood, heart, liver and kidney was measured