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Molecules 2012, 17, 14795-14809; doi:10.3390/molecules171214795 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Telomerase Reverse Transcriptase (TERT) is a Therapeutic Target of Oleanane Triterpenoid CDDO-Me in Prostate Cancer Yongbo Liu 1, Xiaohua Gao 1, Dorrah Deeb 1, Ali S. Arbab 2 and Subhash C. Gautam 1,* 1 Department of General Surgery, Henry Ford Health System, Detroit, MI 48202, USA; E-Mails: bliu1@ (B.L.); tgao1@ (X.G.); ddeeb1@ (D.D.) 2 Department of Radiology, Henry Ford Health System, Detroit, MI 48202, USA; E-Mail: sali1@ * Author to whom correspondence should be addressed; E-Mail: sgautam1@; Tel.: +1-313-874-6998. Received: 9 November 2012; in revised form: 20 November 2012 / Accepted: 6 December 2012 / Published: 11 December 2012 Abstract: Methyl-2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oate (CDDO-Me) is an synthetic oleanane triterpenoid with strong antiprolifertive and proapoptotic activities in cancer cells. However, the effect of CDDO-Me on human telomerase reverse transcriptase (hTERT) and its telomerase activity in prostate cancer cells has not been studied. We investigated the role of hTERT in mediating the anticancer activity of CDDO-Me in prostate cancer cells in vitro and in vivo. The inhibition of cell proliferation and induction of apoptosis by CDDO-Me in LNCaP and PC-3 prostate cancer cell lines was associated with the inhibition of hTERT gene expression, hTERT telomerase activity and a num