通过细胞受体代谢生物素化进行图像分析Metabolic biotinylation of mammalian cell receptors for imaging.pdf

中国试剂网 通过细胞受体代谢生物素化进行图像分析 Metabolic biotinylation of mammalian cell receptors for imaging Metabolic biotinylation of mammalian cell receptors for imaging Bakhos A. Tannous , btannous@, Massachusetts General Hospital and Harvard Medical School Jan Grimm , jgrimm@, Massachusetts General Hospital and Harvard Medical School, Katherine F. Perry , katiefperry@, Massachusetts General Hospital and Harvard Medical School Ralph Weissleder , weissleder@, Massachusetts General Hospital and Harvard Medical School Xandra O. Breakefield , breakefield@, Massachusetts General Hospital and Harvard Medical School Related Journal Article Information Manuscript: NMETH-MT02031 Journal: Nature Methods Article Title: Metabolic biotinylation of cell surface receptors for in vivo imaging Introduction A versatile technique for labeling and imaging cells in culture and in vivo is presented. A metabolically biotinylated reporter protein is expressed on the cell surface which can then be tracked with any imaging agent coupled to streptavidin. A model recombinant reporter protein incorporates the prokaryotic Propionibacterium shermanii 1.3S transcarboxylase domain biotin acceptor peptide (BAP) between an N-terminal signal sequence and the platelet derived growth factor receptor transmembrane domain ™1. The BAP sequence provides a means to track any protein and the BAP-TM reporter allows for non-invasive real time imaging of any cell type transduced to express it in culture or in vivo using a variety of techniques including 中国试剂网 optical1,2, magnetic resonance1,3 and positron emission tomogrophic imaging4. Materials R