通过细胞受体代谢生物素化进行图像分析Metabolic biotinylation of mammalian cell receptors for imaging.pdf
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通过细胞受体代谢生物素化进行图像分析
Metabolic biotinylation of mammalian cell receptors for imaging
Metabolic biotinylation of mammalian cell receptors for imaging
Bakhos A. Tannous , btannous@, Massachusetts General Hospital
and Harvard Medical School
Jan Grimm , jgrimm@, Massachusetts General Hospital and
Harvard Medical School,
Katherine F. Perry , katiefperry@, Massachusetts General Hospital and
Harvard Medical School
Ralph Weissleder , weissleder@, Massachusetts General Hospital and
Harvard Medical School
Xandra O. Breakefield , breakefield@, Massachusetts General
Hospital and Harvard Medical School
Related Journal Article Information
Manuscript: NMETH-MT02031
Journal: Nature Methods
Article Title: Metabolic biotinylation of cell surface receptors for in vivo imaging
Introduction
A versatile technique for labeling and imaging cells in culture and in vivo is presented.
A metabolically biotinylated reporter protein is expressed on the cell surface which
can then be tracked with any imaging agent coupled to streptavidin. A model
recombinant reporter protein incorporates the prokaryotic Propionibacterium
shermanii 1.3S transcarboxylase domain biotin acceptor peptide (BAP) between an
N-terminal signal sequence and the platelet derived growth factor receptor
transmembrane domain ™1. The BAP sequence provides a means to track any protein
and the BAP-TM reporter allows for non-invasive real time imaging of any cell type
transduced to express it in culture or in vivo using a variety of techniques including
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optical1,2, magnetic resonance1,3 and positron emission tomogrophic imaging4.
Materials
R
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