红豆杉多糖的生物活性及其提取纯化研究进展.PDF

·综 述· 红豆杉多糖的生物活性及其提取纯化研究进展 1 1 1 1,2* 叶佐武 ，杨秀丽 ，石佳娜 ，黄萍 (1.浙江省立同德医院药剂科，杭州 310012；2.浙江省中医药研究院，杭州 310007) 摘要：目的 介绍红豆杉多糖的生物活性及其提取纯化研究进展，为红豆杉资源的进一步利用提供依据。方法 通过查 阅近 10 年的国内外文献，对红豆杉多糖的生物活性及其提取纯化进行概述。结果 红豆杉多糖具有免疫调节、抗肿瘤、 减轻缺血再灌注导致的心肌损伤等作用；红豆杉多糖提取纯化工艺取得一定的进展，可采用组织破碎提取法、超声波提 取等方法进行提取，并利用树脂法、双氧水法等方法进行纯化。结论 红豆杉多糖的研究和开发利用具有广阔的前景， 但其提取、分离和纯化技术有待进一步研究。 关键词??红豆杉多糖；生物活性；纯化 中图分类号：R284.2 文献标志码：A 文章编号：1007-7693(2013)05-0549-04 The Development of Biological Activity and Study on Extraction and Purification for Taxus Polysaccharides YE Zuowu1, YANG Xiuli1, SHI Jiana1, HUANG Ping1,2*(1.Tongde Hospital of Zhejiang Province, Hangzhou 310012, China; 2.Traditional Chinese Medicine Research Institute of Zhejiang Province, Hangzhou 310007, China) ABSTRACT: OBJECTIVE To review the development of study on biological activity, extraction and purification for Taxus polysaccharides. METHODS To summarize the biological activity and the technique of extraction and purification of Taxus polysaccharides by reviewing the domestic and foreign articles in the past ten years. RESULTS There are multiple functions for Taxus polysaccharides such as adjusting immunity, anti tumor, alleviating ischemia reperfusion injury on myocardium, and so on. A certain development has been approached on the technique of extraction and purification for Taxus polysaccharides. The extraction methods of tissue crush and ultrasonic wave are recommended. And purification ways of peucine and hydrogen peroxide are selectable. CONCLUSION There are broad prospects on the study and exploitation of Taxus polysaccharides. The techniques of extraction, separation and purification, however, are worth further research. KEY WORDS: Taxus polysaccharides; biological activity; purification 多糖是广泛存在于有机体内的一种重要的信 植物多糖最重要的药理