试析灯盏花颗粒主要药效学试验.doc

试析灯盏花颗粒主要药效学试验 试析灯盏花颗粒主要药效学试验 日期： 08月25日 【摘要 目的 应用二道血流速度流量仪超声探头测定大鼠颈总动脉 (结扎颈外动脉) 以反映云南植物药业有限公司提供的灯盏花颗粒对脑血流量的影响；记录小鼠断头后张口喘气时间，观察灯盏花颗粒的抗脑缺血功能。方法 采用Born方法测定灯盏花颗粒在体内外对AA、ADP及PAF诱导的家兔血小板聚集功能的影响；运用电刺激大鼠颈动脉血栓形成方法，评价灯盏花颗粒对血栓形成的阻抑功能。结果 灯盏花颗粒显著增加脑血流量并延长小鼠断头后喘气时间；灯盏花颗粒在体内外均明显抑制AA、ADP及PAF诱导的血小板聚集功能的同时还显著延长电刺激大鼠颈动脉的血栓形成时间。结论 灯盏花颗粒可能通过抗血小板和抗血栓形成等机制来明显增加脑组织的血供，这对保护缺血性脑组织是十分有利的。 【 灯盏花颗粒 血小板聚集 血栓形成 脑血流量 Pharmacokinetic experiment of Erigeron breviscapus pellets 【Abstract Objective The arteria carotis externa was ligated， and arteria carotis interna was kept opening The left carotid artery was exposed and placed over an ultrasonic flow probe. The brain flow volume was recorded with an ultrasonic volume meter. The pursiness time of mice was observed to evaluate the effect of Erigeron breviscapus pellets on cerebral ischemia. Methods Born’s method was used to investigate effects of Erigeron breviscapus pellets on rabbit platelet aggregation induced by adenosine diphosphate (ADP)， arachidonic acid (AA)， and platelet-activating factor (PAF)， in vitro or in vivo. Antithrombotic effects of Erigeron breviscapus pellets was monitored according to Peter’s method. Results The results showed that Erigeron breviscapus pellets significantly increased brain blood flow and prolonged mouse pursiness time; Erigeron breviscapus pellets markedly inhibited ADP-， AA-， or PAF-induced platelet aggregation in vitro or in vivo， and prolonged the occlusion time of electrically-stimulated carotid artery.Conclusion It is suggested that Erigeron breviscapus pellets improved cerebral ischemia and hypoxia due to its antiplatelet and antithrombotic effects. It is obviously advantageous to protect ischemic brain tissue. 【Key words erigeron breviscapus pellets platelet aggregation thrombosis brain blood flow 血小板聚集性增高在血栓栓塞性疾病的发生发展过程中起着重要功能［1］。灯盏花［Erigeron breviscapus (Vant.)Hand.-Mazz］是云南省特有的民族药用植物， 民间主要用其防治中风偏瘫等疾病。灯盏花颗粒的主要有效组分是灯盏花总黄酮 (Erigeron reviscapus flavones)， 主要成份有灯盏花素、芹菜素、4′-羟基黄芩素等［2］。灯盏花颗粒临床主要用于防治缺