电穿孔过程简介.ppt

Hands-On ;使用样品杯--离体电穿孔In-Vitro Electroporation using Cuvettes;Check List: Safety stand, electroporation buffer (with DNA), cuvettes, pipettes. 清单： 1、安全操作池 2、带DNA的缓冲液 3、样品杯 4、移液管 ;Remove the cuvette from package 将样品杯从包装内取出;Pipette sample from 50 ml tube 吸取样品50ml;Load sample into cuvette 将样品注入样品杯;Place cuvette into safety stand, making sure metal contacts of safety stand touches the cuvette. Use the black roller to adjust the gap between metal contacts. 将样品杯放入安全操作池（注意两个金属表面方向一致），旋紧。;Close lid of safety stand before pulsing 盖上安全盖;Make sure all cables are connected well. Push start on the instrument to deliver pulse. 在确定电缆连接正确后，按下开始，启动脉冲;After pulsing, sample will foam depending on the buffer type and electrical parameters. 脉冲后，样品有泡沫（与缓冲液的类型和电极??形状有关）;Plate the sample into plates for further assay. 将样品移至平板，用于下一步的研究;That’s AllFOLKS!