lc-ms课件.pdf

g g g n n n i i i n n n i i i a a a r r r T T T S Practical S S M M M C Techniques for C C L L L LC/MS U U U Z Z Z Analysis D D D A A A M M M I I I Robert Classon H H H S rjclasson@ S S Revised 1/12/2004 This presentation is an introduction to LCMS. It includes optimization and applications 1 LC/MS seminar overview g g g n ? Getting the most out of each ionization n n i i i n n n i i technique i a a a r r r ? Practical considerations with LC/MS T T T S S S ? Developing LC/MS methods M M M C C C L L L U U U Z Z Z D D D A A A M M M I I I H H H S S S 2 Is LC/MS Ready for HPLC? g g g n n n i i i n n n i i i a a a r r r T T T S S S M M M C C C L L L U U U Z Z Z D D D A A A M M M I I I H H H S S S Since this MS is intended for quantitative use as well as identification, it i important to be able to achieve good quantitative results. Here we show reproducibility on both UV and MS to show comparable results for a pharmaceutical type product (reserpine). Notice that the signal to noise from the MS is considerably better than the U