均相时间分辨荧光免疫分析综述.pdf

Chem. Rev. 2010, 110, 2729–2755 2729 Lanthanide Luminescence for Biomedical Analyses and Imaging Jean-Claude G. Bu¨nzli* ´ Laboratory of Lanthanide Supramolecular Chemistry, Ecole Polytechnique Fe´de´rale de Lausanne (EPFL), BCH 1402, CH-1015 Lausanne, Switzerland, and Department of Advanced Materials Chemistry, WCU Center for Next Generation Photovoltaic Systems, Korea University, Sejong Campus, 208 Seochang, Jochiwon, Chung Nam 339-700, Republic of Korea Received November 5, 2009 Contents 1. Introduction 2729 2. Lanthanide Luminescence 2730 2.1. Basic Facts 2730 2.2. Sensitization by Organic Ligands 2732 2.3. Information Gained from Lanthanide 2732 Luminescent Probes 2.3.1. Hydration Numbers 2732 2.3.2. Energy Transfer and FRET Experiments 2733 2.3.3. Collisional (Stern-Volmer) Quenching 2734 2.4. Designing Efficient Lanthanide Luminescent 2735 Bioprobes (LLBs) 3. Lanthanide Bioconjugates 2736 4. Bioanalyses with Lanthanide Luminescent Probes 2737 Jean-Claude Bu¨nzli is an active researcher in the field of co-ordination, 4.1. Heterogeneous Immunoassays