最新含羟基的阳离子聚合物的合成及其基因传递性能的研究.pdf
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目录 硕士论文
3.2实验方法……………………………………………………………………………25
3.2.1
LB培养基的配制………………………………………………………。25
3.2.2
3.2.3质粒DNA的扩增………………………………………………………25
3.2.4含羟基的交联PEI聚合物/DNA复合物的制备…………………………26
3.2.5胶体电泳实验……………………………………………………………27
3.2.6体外转染实验……………………………………………………………27
3.2.7转染效果的表征……………………………………………………………………27
3.2.8毒性实验………………………………………………………………..27
3.2.9复合物粒度分析实验……………………………………………………28
3.3结果与讨论…………………………………………………………………………28
3.3.1胶体电泳实验……………………………………………………………28
3.3.2体外转染实验……………………………………………………………29
3.3.3毒性实验………………………………………………………………一33
3.3.4复合物粒度分析实验……………………………………………………35
3.3.5含羟基的交联PEI聚合物的结构与性能分析……………………………35
3.4本章小结…………………………………………………………………………….36
4结论……………………………………………………………………….………………………….37
致 谢……………………………………………………………………………………………….38
参考文献…………………………………………………………………………………………….39
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