无机化学（张天蓝）Stable Prenucleation Calcium Carbonate Clusters.pdf

/cgi/content/full/322/5909/1819/DC1 Supporting Online Material for Stable Prenucleation Calcium Carbonate Clusters Denis Gebauer, Antje Völkel, Helmut Cölfen* *To whom correspondence should be addressed. E-mail: coelfen@mpikg.mpg.de Published 19 December 2008, Science 322, 1819 (2008) DOI: 10.1126/science.1164271 This PDF file includes: Materials and Methods SOM Text Figs. S1 to S15 Table S1 References Stable Prenucleation Calcium Carbonate Clusters Denis Gebauer, Antje Völkel, Helmut Cölfen* Max Planck Institute of Colloids and Interfaces, Research Campus Golm, Am Mühlenberg 1, D-14424 Potsdam, Germany, *coelfen@mpikg.mpg.de Supporting Online Material 1 Materials and Methods 1.1 Principal Setup We utilize a commercial, computer controlled titration system manufactured by Metrohm. The titration software is tiamo 1.1. The setup consists of two titration devices Titrando 809 controlling four dosing devices 800 Dosino that operate an 807 Dosing Unit, respectively. The 807 Dosing Unit incorporates a 2 mL glass cylinder, with a depletion divided into 10,000 steps resulting in a minimal dose-able volume of 0.2 μL. We utilize two pH-electrodes (Metrohm No. 6.0227.100 and No. 6.0256.100) and one calcium ion selective electrode (Metrohm No. 6.0508.110). For calcium potential measurements, the reference system of pH-electrodes is utilized. 1.2 Preparation of Solutions All solutions are prepared using Millipore water that is purged with nitrogen overnight in order to remove residual carbon dioxide. The 10 mM sodium hydroxide solution used in constant-pH titrati