一种膜荚黄芪凝集素的分离纯化研究.pdf

∀ 71 ∀ 中草药 Chinese Traditional and Herbal Drugs 1 5 2010 5 1 2 1* 1 2 朱利芬 , 闫巧娟 , 江正强 ,Kumar Narasimha , 黄林华 ( 1 , 100083; 2 , 100083) : ConASepharose 4B , SDSPA E 4 4 Superdex 75 315 10 335 10 , , 107% ,, , 3919 U/ mg : ; ;; :R2842 :A : 2010 Isolation and purification of a lectin from roots of Astrag alus membranaceus 1 2 1 1 2 ZHU Lifen , YAN Qiaojuan , JIAN Zhengqiang , KUMAR Narasimha , HUAN Linhua ( 1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2 College of Engineering, China Agricultural University, Beijing 100083, China) Abstract: Objective T o isolate and purify a lectin from the roots of A stragalus membranaceus Methods T he protein was purified using a combination of 20% ! 60% ammonium sulfate fraction and ConASepharose 4B affinity chromatography Results The purified protein appeared as a single band with 4 molecular mass of 315 10 on SDSPA E and the relative molecular mass was estimated by gel filtration 4 on a calibrated Superdex 75 column with apparent molecular weight of 335 10 This lectin was a glyco protein with a neutral carbohydrate content of 107% Conclusion A lectin is isolated and purified from the roots of A membranaceus for the first time It is a monomer glycoprotein and its specific activity is 3919 U/ mg Key words: A stragalus membranaceus (Fisch) Bunge; lectin; purification; affinity chromatography ( B